Thermo Scientific™ HpaII

The HpaII restriction enzyme recognizes C^CGG sites and cuts best at 37°C in Tango buffer.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER0512

HPAII 10U/UL 5000U

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 1.51950kg



Product Code. 10171170

Quantity Price
1 £ 102.0 / 5000 units
EU Stock 7
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Description and Specification

Specification

Product Name HpaII
Concentration 10U/µL
Units 5,000U

5'...C▵C G G...3'

3'...G G C▵C...5' Conditions for 100% Activity:

  • 1X Buffer Tango™: 33mM Tris-acetate (pH7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • HpaII is supplied in: 10mM Tris-HCl (pH7.4 at 25°C), 100mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: completely overlaps —blocked
  • EcoKI: never overlaps —no effect
  • EcoBI: never overlaps —no effect

Compatible Ends:

  • Bsp119I, Bsu15I, Hin1I, MaeII, NarI, Psp1406I, SsiI, TaqI, XmiI