Thermo Scientific™ HincII (HindII)

The HincII (HindII) restriction enzyme recognizes GTY^RAC sites and cuts best at 37°C in Tango buffer (Isoschizomers: HindII).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER0491

HINCII (HINDII) 10U/UL 500U

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.00900kg



Product Code. 10389529

Quantity Price
1 £ 24.3 / 500 units
EU Stock 42
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Description and Specification

Specification

Product Name HincII (HindII)
Concentration 10U/µL
Units 500U

5'...G T Y▵R A C...3'

3'...C A R▵Y T G...5'

Conditions for 100% Activity:

  • 1X Buffer Tango™: 33mM Tris-acetate (pH7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • HincII is supplied in: 10mM Tris-HCl (pH 7.5 at 25°C), 200mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: may overlap —cleavage impaired
  • EcoKI: may overlap —blocked
  • EcoBI: may overlap —blocked