Thermo Scientific™ Genomic DNA Purification Kit

Product Code. 10571031

Brand: Thermo Scientific™ K0512

Description

Isolate genomic DNA from various sources, including whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues, with this DNA precipitation system.

Genomic DNA Purification Kit is a simple and rapid system for high quality genomic DNA purification from various sources including: whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues.

The kit is based on selective detergent-mediated DNA precipitation from crude lysate. The entire procedure is rapid only 20 to 25 minutes with a typical yield of 2 to 10 μg genomic DNA from 0.2 mL of blood.

High molecular weight genomic DNA purified with the kit is suitable for direct use in all common molecular biology applications: PCR, FastDigest and conventional restriction digestion, cloning, DNA sequencing, and Southern blot analysis.

Highlights

• Efficient—2 to 10 μg of genomic DNA from 0.2 mL of blood
• Fast—procedure takes approximately 20 minutes
• Universal—purifies genomic DNA from various sources
• Flexible—easy to scale up and down
• Safe—does not involve phenol extraction

Specifications

• Content And Storage: • 40 mL Lysis Solution; room temperature
  • 8 mL Precipitation Solution (concentrated); room temperature
  • 10 mL NaCl Solution; room temperature
  
  
• Isolation Technology: Organic Extraction
• Sample Type: Bacteria, Cells, Plant, Serum, Tissue, Whole Blood, Blood
• Elution Volume: 20 to 100 μL
• Final Product Type: gDNA
• For Use With (Application): PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Quantity: 100 Preps
• Shipping Condition: Room Temperature
• Test Time: 25 min.
• Starting Material Amount: Bacteria: ≤20 mg
  Cells: ≤10⁶
  Plant: ≤100 mg
  Tissue: ≤30 mg
  Serum: ≤200 μL
  Whole Blood: ≤200 μL

Safety and Handling

Product Identifier

• Genomic DNA Purification Kit

Signal Word

• Danger

Hazard Category

• LONG-TERM AQUATIC HAZARD Chronic 2
• ACUTE AQUATIC Acute 1
• Serious eye damage/eye irritation Category 1
• Skin corrosion/irritation Category 2
• Specific target organ toxicity after repeated exposure Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H315-Causes skin irritation.
• H318-Causes serious eye damage.
• H335-May cause respiratory irritation.
• H373-May cause damage to organs.
• H400-Very toxic to aquatic life.
• H411-Toxic to aquatic life with long lasting effects.

Precautionary Statement

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P271-Use only outdoors or in a well-ventilated area.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P310-Immediately call a POISON CENTER/doctor/.
• P312-Call a POISON CENTER/doctor/if you feel unwell.
• P314-Get medical advice/attention if you feel unwell.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P391-Collect spillage.
• P403+P233-Store in a well-ventilated place. Keep container tightly closed.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : N,N,N-trimethyl-1-tetradecanaminium bromide, Hexadecyltrimethylammonium bromide

For Research Use Only. Not for use in diagnostic procedures.