Thermo Scientific™ GeneJET Viral DNA/RNA Purification Kit

Product Code. 11951411

Brand: Thermo Scientific™ K0821

Description

Perform silica membrane-based viral nucleic acid purification from human and mammalian plasma, serum, blood, and cell-free samples.

GeneJET Viral DNA and RNA Purification Kit is designed for rapid and efficient purification of high quality viral nucleic acids from various human and mammalian liquid samples, and is compatible with common sample preservatives like EDTA, citrate, and heparin.

The kit utilizes a silica-based membrane technology in the form of a convenient spin column. Viral nucleic acids from lysed samples bind to the column membrane while impurities are effectively removed during subsequent washing and centrifugation steps. Ready-to-use nucleic acids are eluted from the column.

The purified viral nucleic acids are free of proteins, nucleases, and other contaminants or inhibitors of downstream applications. Isolated DNA and RNA can be directly used in PCR, qPCR, or other nucleic acid based assays.

Highlights:

• Efficient purification of viral DNA and RNA from human and mammalian plasma, serum, blood, and cell-free samples
• Linear yields from a wide range of viral titers
• Tested with a variety of DNA and RNA viruses (HIV, HBV, HCV, HPV, CMV, EBV)
• Downstream compatibility with (RT)-PCR, (RT)-qPCR, and other nucleic acid based assays.

Specifications

• Column Type: Spin Column
• Content And Storage: • 2 x 1.4 mL Column Preparation Liquid; room temperature
  • 12 mL Lysis Solution; room temperature
  • 25 mL Wash Buffer 1 (concentrated); room temperature
  • 11 mL Wash Buffer 2 (concentrated); room temperature
  • 3 x 1.25 mL Eluent; room temperature
  • 50 Spin Columns with Wash Tubes; room temperature
  • 4 x 50 Wash Tubes; room temperature
  • 2 x 1.3 mL Proteinase K; –20°C
  • 1 vial Carrier RNA; –20°C
  
  
• Isolation Technology: Silica Spin Column
• Scale: Mini
• Sample Type: Milk, Plasma, Saliva, Serum, Swabs (Nasal, Buccal, or Urogenital), Urine, Whole Blood, Virus
• Elution Volume: 50 μL
• Final Product Type: Viral DNA and RNA
• For Use With (Application): RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Quantity: 50 Preps
• Shipping Condition: Room Temperature
• Test Time: 30 min.
• Yield: 50 μg (Binding capacity)
• Starting Material Amount: 200 μL

Safety and Handling

Product Identifier

• GeneJET Viral DNA/RNA Purification Kit

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3
• Serious eye damage/eye irritation Category 1
• Serious eye damage/eye irritation Category 2
• Respiratory sensitiser Category 1
• Skin corrosion/irritation Category 2

Hazard Statement

• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H318-Causes serious eye damage.
• H319-Causes serious eye irritation.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P284-¢In case of inadequate ventilation! wear respiratory protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P330-Rinse mouth.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Guanidine hydrochloride, Proteinase

For Research Use Only. Not for use in diagnostic procedures.