Thermo Scientific™ GeneJET Plasmid Maxiprep Kit

Product Code. 11551645

Brand: Thermo Scientific™ K0491

Description

Thermo Scientific GeneJET Plasmid Maxiprep Kit, sufficient for 25 preps, utilizes silica-based membrane technology in the form of convenient spin column.

Thermo Scientific GeneJET Plasmid Maxiprep Kit, sufficient for 25 preps, utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers greater than 400 ng/μL of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

Highlights

• Efficient—high yields up to 750 μg of high quality plasmid DNA from 250 mL of bacterial culture
• High concentration of DNA purified— >400 ng/μL
• Convenient—spin column format, centrifuge or vacuum protocols
• Fast—procedure takes 60 minutes, considerably faster than using gravity flow columns

Applications

Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:

• Transfection of robust cell lines
• FastDigest restriction enzyme digestion
• Automated fluorescent sequencing
• PCR
• Cloning
• Transformation
• In vitro transcription

Specifications

• Concentration: 400 ng/μL
• Column Type: Spin Column
• Format: Spin Column
• Isolation Technology: Silica Column
• Scale: Maxi
• Sample Type: Bacteria
• For Use With (Application): Real-Time Quantitative PCR (qPCR), Transfection, Cloning, Sequencing, In Vitro Transcription, Transformation, Nucleic Acid Labeling, PCR
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• No. of Reactions: 10 Preps
• Prep Scale: > 200 μg (Large-Scale) Plasmid DNA
• Product Line: GeneJET
• Product Type: Plasmid MaxiPrep Kit
• Quantity: 10 preps
• Target: Plasmid DNA
• Test Time: 60 min.

Safety and Handling

Product Identifier

• GeneJET Plasmid Maxiprep Kit

Signal Word

• Warning

Hazard Category

• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2

Hazard Statement

• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.

Precautionary Statement

• P264-Wash hands thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P330-Rinse mouth.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P362+P364-Take off contaminated clothing and wash it before reuse.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : sodium lauryl sulphate, sodium hydroxide, acetic acid, guanidine hydrochloride

For Research Use Only. Not for use in diagnostic procedures.