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Thermo Scientific™ GeneJET™ Plasmid Maxiprep Kit

Perform large-scale isolation of high-quality plasmid DNA from recombinant E. coli cultures with this kit.

Brand:  Thermo Scientific™ K0492

Code : 88

Additional Details : Weight : 7.50240kg Transport : UN number : 3316 Chem class : 1 Pack group : III


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Product Code. 11561645

  • £255.00 / 25 preps


EU Stock 8
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Description

Description

The kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 750μg of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription and transfection of robust cell lines.
  • Efficient: High yields up to 750μg of high quality plasmid DNA from 250mL of bacterial culture

  • High concentration of DNA purified: 400ng/μl

  • Convenient: Spin column format, centrifuge or vacuum protocols

  • Fast: Procedure takes 60min, considerably faster than using gravity flow columns

Includes:

Resuspension Solution, Lysis Solution, Neutralization Solution, Endotoxin Binding Reagent, Wash Solution I (concentrated), Wash Solution II (concentrated), RNase A Solution, Elution Buffer (10mM Tris-HCl, pH 8.5), GeneJET™ Maxi Purification Columns pre-assembled with collection tubes (50mL), Collection tubes (50mL)

Recommended for:

Transfection of robust cell lines, FastDigest®, restriction enzyme digestion, automated fluorescent sequencing, PCR, cloning, transformation,in vitro transcription.

Specifications

Specifications

400ng/µl
Resuspension Solution, Lysis Solution, Neutralization Solution, Endotoxin Binding Reagent, Wash Solution I (concentrated), Wash Solution II (concentrated), RNase A Solution, Elution Buffer (10mM Tris-HCl, pH 8.5), GeneJET™ Maxi Purification Columns pre-assembled with collection tubes (50mL) and Collection tubes (50mL).
Thermo Scientific™ GeneJET Plasmid Maxiprep Kit
Transfection of robust cell lines, FastDigest®, restriction enzyme digestion, automated fluorescent sequencing, PCR, cloning, transformation,in vitro transcription.
25 Preps
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Safety and Handling

Safety and Handling

Product Identifier
  • GeneJET Plasmid Maxiprep Kit

Signal Word
  • Danger

Hazard Category
  • Skin corrosion/irritation Category 1 A
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2

Hazard Statement
  • H319-Causes serious eye irritation.
  • H314-Causes severe skin burns and eye damage.

Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P310-Immediately call a POISON CENTER/doctor/A§.
  • P373-DO NOT fight fire when fire reaches explosives.

Supplemental information
  • MIXTURE LIST-Contains : Sodium lauryl sulfate, Sodium hydroxide,acetic acid, Sodiumlauryl sulfate, Sodium hydroxide