Thermo Scientific™ FspBI (BfaI)

The FspBI (BfaI) restriction enzyme recognizes C^TAG sites and cuts best at 37°C in Tango buffer (Isoschizomers: BfaI, MaeI, XspI).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER1762

RESTRICTION ENZYME, CONVENTIONAL, FSPBI (BFAI),10u/ul, cuts C¬TAG at 37°C (supplied with 1mL of

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.00900kg



Product Code. 10869700

Quantity Price
1 £ 239.0 / Each
EU Stock 1
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Description and Specification

Specification

Product Name FspBI (BfaI)
Concentration 10U/µL
Units 2,500U

More than 80% of the digested DNA fragments can be ligated and more than 95% can be recut in a reaction mixture containing 20 to 40U of T4 DNA ligase/1μg of fragments and 10% PEG at a 5ft.-termini concentration of 0.13μM after a 50-fold overdigestion(3U/μg DNA x 17 hours) with BfuI FspBI (BfaI).

5'...C▵T A G...3'

3'...G A T▵C...5'

Conditions for 100% Activity:

  • 1X Buffer Tango™ :33mM Tris-acetate (pH7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • FspBI is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: may overlap — no effect
  • Dcm: never overlaps — no effect
  • CpG: never overlaps — no effect
  • EcoKI: never overlaps — no effect
  • EcoBI: never overlaps — no effect

Digestion of Agarose-embedded DNA:

  • Minimum 10U of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 4 hours

Note:

FspBI cleavage is impaired by overlappingdam methylation. To avoiddam methylation, use adam-, dcm strain such as GM2163 (#M0099).