Thermo Scientific™ FspAI

The FspAI restriction enzyme recognizes RTGC^GCAY sites and cuts best at 37°C in O buffer.

Brand: Thermo Scientific™

Manufacturer Part Number: ER1661

100 UNIT RESTRICTION ENZYME, CONVENTIONAL, FSPAI,5u/ul, cuts RTGC¬GCAY at 37°C (supplied with 1mL

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.01000kg

Product Code. 10304430

Quantity Price
1 £79.00 / 100 units
EU Stock 6
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Description and Specification


Product Name FspAI
Concentration 5U/µL
Units 100U

More than 80% of the digested DNA fragments can be ligated and more than 90% can be recut at a 5ft.-termini concentration of 0.2μM after a 50-fold overdigestion (3U/μg DNA x 17 hours) with FspAI.

5'...R T G C▵G C A Y...3'

3'...Y A C G▵C G T R...5'

Conditions for 100% Activity:

  • 1X Buffer O: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 100mM NaCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • FspAI is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol


  • Dam: never overlaps — no effect
  • Dcm: never overlaps — no effect
  • CpG: completely overlaps — blocked
  • EcoKI: may overlap — effect not determined
  • EcoBI: may overlap — effect not determined


Low salt, high glycerol (>5%) concentrations, pH >8.0 or a large excess of enzyme may result in star activity.