First Strand cDNA Synthesis Kit

Product Code. 10774691

Brand: Thermo Scientific K1612

Description

Thermo Scientific™ Fermentas First Strand cDNA Synthesis Kit is a complete system for the efficient synthesis of first strand cDNA from RNA templates.

Perform efficient synthesis of first strand cDNA from RNA templates with the complete system in Thermo Scientific™ Fermentas First Strand cDNA Synthesis Kit.

The kit utilizes the recombinant M-MuLV Reverse Transcriptase which exhibits lower RNase H activity than AMV reverse transcriptase. The recombinant RiboLock RNase Inhibitor, supplied with the kit effectively protects RNA template from degradation. The oligo(dT)₁₈ anneals selectively on the poly(A) tail of mRNA. Random hexamer primers do not require the presence of poly(A). Therefore, they can be used for transcription of the 5'-end regions of mRNA or cDNA synthesis using RNA without poly(A) tail, e.g., micro RNAs. Gene-specific primers may also be used with the kits.The first strand of cDNA can be directly used as a template in PCR (see RT-PCR protocol) or in second strand cDNA synthesis (see protocol).

• Efficient synthesis of first strand cDNA up to 9kb
• Reaction temperature 37°C
• Supplied with the recombinant RiboLock RNase Inhibitor
• Complete—oligo(dT)₁₈ and random hexamer primers included with the kit

Recommended for:

First strand cDNA synthesis for RT-PCR and real-time RT-PCR; Construction of cDNA libraries; Generation of probes for hybridization; aRNA synthesis.

Specifications

• Product Type: Fermentas™ First Strand cDNA Synthesis Kit
• Sufficient For: 100 reactions
• For Use With (Application): RT-PCR