Thermo Scientific™ Esp3I (BsmBI)

The Esp3I (BsmBI) restriction enzyme recognizes CGTCTC(1/5)^ sites and cuts best at 37°C in Tango (+DTT) buffer (Isoschizomers: BsmBI).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER0452

ESP3I (BSMBI) 10U/UL 1000U

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 1.51950kg



Product Code. 10859360

Quantity Price
1 £ 243.0 / 1000 units
EU Stock 23
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Description and Specification

Specification

Product Name Esp3I (BsmBI)
Concentration 10U/µL
Incubator Temperature 37°C
Quantity 1000U

5'...C G T C T C (N)1▵...3'

3'...G C A G A G (N)5▵...5' Conditions for 100% Activity:

  • [1X Buffer Tango] + DTT: [33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA] + 1.0mM DTT
  • Incubate at 37°C

Storage Buffer:

  • Esp3I is supplied in: 10mM potassium phosphate (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.5mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with Esp3I, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps-no effect
  • Dcm: never overlaps-no effect
  • CpG: completely overlaps-blocked
  • EcoKI: never overlaps-no effect
  • EcoBI: may overlap-effect not determined

Digestion of Agarose-embedded DNA:

  • Minimum 5U of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours

Note:

The enzyme requires DTT. Freshly made DTT should be added to the reaction buffer. Esp3I cleaves downstream of its recognition site and can generate any desired 4 base 5'-overhangs. This feature is useful for PCR product cloning.