missing translation for 'onlineSavingsMsg'
Learn More

Thermo Scientific™ Esp3I (BsmBI)

The Esp3I (BsmBI) restriction enzyme recognizes CGTCTC(1/5)^ sites and cuts best at 37°C in Tango (+DTT) buffer (Isoschizomers: BsmBI).

Brand:  Thermo Scientific™ ER0452

243.48 GBP valid until 2018-08-31
Use promo code "13204" to get your promotional price.



Code : 88

Additional Details : Weight : 1.51950kg

 View more versions of this product

Product Code. 10859360

  • £261.81 / 1000 units


EU Stock 12
Add to basket

Description

Description

5'...C G T C T C (N)1▵...3'

3'...G C A G A G (N)5▵...5' Conditions for 100% Activity:

  • [1X Buffer Tango] + DTT: [33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA] + 1.0mM DTT
  • Incubate at 37°C

Storage Buffer:

  • Esp3I is supplied in: 10mM potassium phosphate (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.5mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with Esp3I, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps-no effect
  • Dcm: never overlaps-no effect
  • CpG: completely overlaps-blocked
  • EcoKI: never overlaps-no effect
  • EcoBI: may overlap-effect not determined

Digestion of Agarose-embedded DNA:

  • Minimum 5U of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours

Note:

The enzyme requires DTT. Freshly made DTT should be added to the reaction buffer. Esp3I cleaves downstream of its recognition site and can generate any desired 4 base 5'-overhangs. This feature is useful for PCR product cloning.

Specifications

Specifications

Esp3I (BsmBI)
10U/μL
37°C
1000U
Videos
Special Offers

Special Offers

  • Big discounts are available on a range of popular products including chemicals, lab essentials, equipment, consumables, life science and safety products.
    : 13204

    2018-08-31

    *Discount will apply to qualifying orders received by Fisher Scientific between 04/06/2018 and 31/08/2018. Promotion code must be presented at the time of order. Discount applies to the list price in effect at the time the order is received. Promotion cannot be combined with other discounts or promotions. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.

    Terms & Conditions