Thermo Scientific™ Esp3I (BsmBI)

The Esp3I (BsmBI) restriction enzyme recognizes CGTCTC(1/5)^ sites and cuts best at 37°C in Tango (+DTT) buffer (Isoschizomers: BsmBI).

Brand: Thermo Scientific™

Manufacturer Part Number: ER0451


UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.00800kg

Product Code. 10374280

Quantity Price
1 £55.25 / 200 units
EU Stock 13
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Description and Specification


Product Name Esp3I (BsmBI)
Concentration 10U/µL
Incubator Temperature 37°C
Quantity 200U

5'...C G T C T C (N)1▵...3'

3'...G C A G A G (N)5▵...5' Conditions for 100% Activity:

  • [1X Buffer Tango] + DTT: [33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA] + 1.0mM DTT
  • Incubate at 37°C

Storage Buffer:

  • Esp3I is supplied in: 10mM potassium phosphate (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.5mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with Esp3I, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps-no effect
  • Dcm: never overlaps-no effect
  • CpG: completely overlaps-blocked
  • EcoKI: never overlaps-no effect
  • EcoBI: may overlap-effect not determined

Digestion of Agarose-embedded DNA:

  • Minimum 5U of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours


The enzyme requires DTT. Freshly made DTT should be added to the reaction buffer. Esp3I cleaves downstream of its recognition site and can generate any desired 4 base 5'-overhangs. This feature is useful for PCR product cloning.