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Thermo Scientific™ Eco52I (EagI)

The Eco52I (EagI) restriction enzyme recognizes C^GGCCG sites and cuts best at 37°C in its own unique buffer (Isoschizomers: BseX3I, BstZI, EagI, EclXI).

Brand:  Thermo Scientific™ ER0332

Code : 88

Additional Details : Weight : 0.01000kg

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Product Code. 10319339

  • £244.12 / 2500 units

EU Stock 5
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5'...C▵G G C C G...3'

3'...G C C G G▵C...5'

Conditions for 100% Activity:

  • 1X Buffer Eco52I:10mM Tris-HCl (pH 8.5 at 37°C), 3mM MgCl2, 100mM NaCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • Eco52I is supplied in:10mM Tris-HCl (pH 8.2 at 25°C), 500mM NaCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with Eco52I, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps-no effect
  • Dcm: never overlaps-no effect
  • CpG: completely overlaps-blocked
  • EcoKI: never overlaps-no effect
  • EcoBI: never overlaps-no effect

Digestion of Agarose-embedded DNA:

  • Minimum 5U of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours

Compatible Ends:

  • Bsp120I, CfrI, NotI


Eco52I (EagI)