Thermo Scientific™ Eco47I (AvaII)

The Eco47I (AvaII) restriction enzyme recognizes G^GWCC sites and cuts best at 37°C in R buffer (Isoschizomers: AvaII, Bme18I, SinI, VpaK11BI).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER0311

ECO47I (AVAII) 10U/UL 800U

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 10213160

Quantity Price
1 £ 14.719999999999999 / 800 units
EU Stock 29
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Description and Specification

Specification

Product Name Eco47I (AvaII)
Concentration 10U/µL
Incubator Temperature 37°C
Quantity 800U

5'...G▵G W C C...3'

3'...C C W G▵G...5'

Conditions for 100% Activity:

  • 1X Buffer R:10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl2, 100mM KCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • Eco47I is supplied in:10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with Eco47I, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps-no effect
  • Dcm: may overlap-blocked
  • CpG: may overlap-blocked
  • EcoKI: never overlaps-no effect
  • EcoBI: never overlaps-no effect

Compatible Ends:

  • Cfr13I, CpoI, Psp5II, SanDI

Note:

Eco47I is blocked by overlappingdcm methylation. To avoiddcm methylation, use adam,dcm- strain such as GM2163 (#M0099).