Thermo Scientific™ Eco147I (StuI)

The Eco147I (StuI) restriction enzyme recognizes AGG^CCT sites and cuts best at 37°C in B buffer (Isoschizomers: AatI, PceI, SseBI, StuI).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER0421

ECO147I (STUI) 10U/UL 1000U

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 1.51950kg



Product Code. 10359889

Quantity Price
1 £ 44.82 / 1000 units
EU Stock 12
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Description and Specification

Specification

Product Name Eco147I (StuI)
Concentration 10U/µL
Quantity 1000U

5'...A G G▵C C T...3'

3'...T C C▵G G A...5'

Conditions for 100% Activity:

  • 1X Buffer B:10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2 and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • Eco147I is supplied in:
  • 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with Eco147I, more than 90% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps — no effect
  • Dcm: may overlap — blocked
  • CpG: never overlaps — no effect
  • EcoKI: never overlaps — no effect
  • EcoBI: may overlap — effect not determined

Digestion of Agarose-embedded DNA:

  • Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours

Note:

Eco147I is blocked by overlappingdcm methylation. To avoiddcm methylation, use adam,dcm- strain such as GM2163 (#M0099).