5'...A G G▵C C T...3'
3'...T C C▵G G A...5'
Conditions for 100% Activity:
Ligation and Recleavage:
Digestion of Agarose-embedded DNA:
Eco147I is blocked by overlappingdcm methylation. To avoiddcm methylation, use adam,dcm- strain such as GM2163 (#M0099).