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Thermo Scientific™ Eco105I (SnaBI)

The Eco105I (SnaBI) restriction enzyme recognizes TAC^GTA sites and cuts best at 37°C in Tango buffer (Isoschizomers: BstSNI, SnaBI).

Brand:  Thermo Scientific™ ER0402

Code : 88

Additional Details : Weight : 0.01000kg

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Product Code. 10349889

  • £244.00 / 3000 units


Estimated Shipment
09-11-2017
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Description

Description

5'...T A C▵G T A...3'

3'...A T G▵C A T...5'

Conditions for 100% Activity:

  • 1X Buffer Tango™:
  • 33mM Tris-acetate (pH7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA.Incubate at 37°C

Storage Buffer:

  • Eco105I is supplied in:10mM Tris-HCl (pH7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 1mM phenylmethylsulfonylfluoride, 0.2mg/ml BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 10-fold overdigestion with Eco105I, more than 80% of the M13mp18 DNA fragments can be ligated in a reaction mixture containing 20-40U of T4 DNA Ligase/1μg of fragments and 10% PEG
  • More than 90% of these can be recut

Methylation Effects:

  • Dam: never overlaps — no effect
  • Dcm: never overlaps — no effect
  • CpG: completely overlaps — blocked
  • EcoKI: never overlaps — no effect
  • EcoBI: never overlaps — no effect

Digestion of Agarose-embedded DNA:

  • Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours

Note:

Greater than 15-fold overdigestion with Eco105I may result in star activity.

Specifications

Specifications

Eco105I (SnaBI)
10U/µL
3,000U