Thermo Scientific™ Eco105I (SnaBI)

The Eco105I (SnaBI) restriction enzyme recognizes TAC^GTA sites and cuts best at 37°C in Tango buffer (Isoschizomers: BstSNI, SnaBI).

Brand: Thermo Scientific™

Manufacturer Part Number: ER0401

ECO105I (SNABI) 10U/UL 600U

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.00900kg

Product Code. 10191070

Quantity Price
1 £52.00 / 600 units
EU Stock 10
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Description and Specification


Product Name Eco105I (SnaBI)
Concentration 10U/µL
Quantity 600U

5'...T A C▵G T A...3'

3'...A T G▵C A T...5'

Conditions for 100% Activity:

  • 1X Buffer Tango™:
  • 33mM Tris-acetate (pH7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA.Incubate at 37°C

Storage Buffer:

  • Eco105I is supplied in:10mM Tris-HCl (pH7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 1mM phenylmethylsulfonylfluoride, 0.2mg/ml BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 10-fold overdigestion with Eco105I, more than 80% of the M13mp18 DNA fragments can be ligated in a reaction mixture containing 20-40U of T4 DNA Ligase/1μg of fragments and 10% PEG
  • More than 90% of these can be recut

Methylation Effects:

  • Dam: never overlaps — no effect
  • Dcm: never overlaps — no effect
  • CpG: completely overlaps — blocked
  • EcoKI: never overlaps — no effect
  • EcoBI: never overlaps — no effect

Digestion of Agarose-embedded DNA:

  • Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours


Greater than 15-fold overdigestion with Eco105I may result in star activity.