Thermo Scientific™ Eam1104I (EarI)

The Eam1104I (EarI) restriction enzyme recognizes CTCTTC(1/4)^ sites and cuts best at 37°C in Tango buffer (Isoschizomers: Bst6I, EarI).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER0232

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.00900kg



Product Code. 10253110

Quantity Price
1 £ 119.0 / 1500 units
EU Stock 21
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Description and Specification

Specification

Concentration 10U/µL
Incubator Temperature 37°C
Product Name Eam1104I (EarI)
Quantity 1,500U

5'...C T C T T C (N)1▵...3'

3'...G A G A A G (N)4▵...5'

Conditions for 100% Activity:

  • 1X Buffer Tango™:
  • 33mM Tris-acetate (pH 7.9 at 37 C), 10 mM Mg-acetate, 66 mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • Eam1104I is supplied in:10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with Eam1104I, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps — no effect
  • Dcm: never overlaps — no effect
  • CpG: may overlap — no effect
  • EcoKI: never overlaps — no effect

Digestion of Agarose-embedded DNA:

  • Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours

Note:

Certain sites in lambda DNA are difficult to cleave with Eam1104I, the same as with its prototype Ksp632I.