5'...C T C T T C (N)1▵...3'
3'...G A G A A G (N)4▵...5'
Conditions for 100% Activity:
Ligation and Recleavage:
Digestion of Agarose-embedded DNA:
Certain sites in lambda DNA are difficult to cleave with Eam1104I, the same as with its prototype Ksp632I.