Thermo Scientific™ DreamTaq DNA Polymerase (5 U/μL)

Product Code.	Color	Quantity	unitSize
11883813	Colorless	5 x 500 Units	Pack of 5
10559120	Colorless	200 Units	200 units
15727496	Colorless	500 Units	500 units
10160790	Colorless	500 Units	500 units
15282086	Colorless	10 x 500 Units	5000 units
10679320	Colorless	20 x 500 Units	Pack of 20
10689320	Green	200 Units	200 units
10776411	Green	500 Units	500 units
15747496	Green	500 Units	500 units
11803823	Green	5 x 500 Units	Pack of 5
15757496	Green	5 x 500 Units	Pack of 5
10262450	Green	20 x 500 Units	Pack of 20

Produktkod. 11883813 Leverantör Thermo Scientific™ Leverantörsnummer EP0703

Beskrivning

Thermo Scientific DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase optimized for all standard PCR applications. It ensures higher sensitivity, longer PCR products, and higher yields compared to conventional Taq DNA polymerase.

Thermo Scientific DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase optimized for all standard PCR applications. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase. DreamTaq DNA Polymerase uses the same reaction setup and cycling conditions as conventional Taq DNA polymerase. Extensive optimization of reaction conditions is not required. It is supplied with optimized DreamTaq Buffer, which includes 20 mM MgCl₂

DreamTaq Green DNA Polymerase is a combination of DreamTaq DNA Polymerase and 10X DreamTaq Green Buffer. The 10X DreamTaq Green Buffer includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with PCR performance and is compatible with downstream applications such as DNA sequencing, ligation, and restriction digestion.

Features

• Robust amplification with minimal optimization
• High yields of PCR products
• Higher sensitivity compared to conventional Taq DNA polymerase
• Amplification of long targets up to 6 kb from genomic DNA and up to 20 kb from viral DNA
• Incorporates modified nucleotides, but does not incorporate dUTP
• Direct loading of PCR product on gel with green format

Applications

• Routine PCR amplification of DNA fragments up to 6 kb
• High throughput PCR
• Genotyping

Notes

• For applications requiring PCR product analysis by absorbance or fluorescence excitation, colorless 10X DreamTaq Buffer (Cat. No. B65) is recommended for purifying PCR products before analysis.

Specifikationer

• Concentration: 5 U/μL
• Content And Storage: • DreamTaq DNA Polymerase (5 U/μL), 5 x 100 μL
  • 10X DreamTaq Buffer with 20 mM MgCl₂, 10 x 1.25 mL
  
  Store at -20°C.
• GC-Rich PCR Performance: Low
• Polymerase: DreamTaq DNA Polymerase
• Reaction Speed: Standard
• Quantity: 5 x 500 Units
• Shipping Condition: Dry Ice
• For Use With (Application): Standard PCR, Genotyping
• Fidelity (vs. Taq): 1X
• Hot Start: No
• Overhang: 3'-A
• Reaction Format: Separate Components
• Color: Colorless

Frequently Asked Questions (FAQs)

What is the error rate of DreamTaq DNA Polymerases?

The error rate of DreamTaq DNA Polymerase is the same as that of Taq DNA Polymerase: 2.2x10^-5 errors per nt per cycle.

For Research Use Only. Not for use in diagnostic procedures.