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Thermo Scientific™ DraI

Cut at TTT^AAA sites with DraI restriction enzyme, which performs best at 37°C in Tango buffer.

Brand:  Thermo Scientific™ ER0223

UNSPSC : 12352204

Code : 88

Additional Details : Weight : 0.01000kg

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Product Code. 10243110

  • £130.00 / 7500 units


Estimated Shipment
14-06-2017
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Specifications

Description

5'...T T T▵A A A...3'

3'...A A A▵T T T...5'

Conditions for 100% Activity:

  • 1X Buffer Tango™:
  • 33mM Tris-acetate (pH7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • DraI is supplied in:10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with DraI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps — no effect
  • Dcm: never overlaps — no effect
  • CpG: never overlaps — no effect
  • EcoKI: may overlap — blocked
  • EcoBI: never overlaps — no effect

Digestion of Agarose-embedded DNA:

  • Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours
Description & Specifications

Specifications

Dral
10U/µL
37°C
7,500U