Thermo Scientific™ DraI

Cut at TTT^AAA sites with DraI restriction enzyme, which performs best at 37°C in Tango buffer.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER0223

RESTRICTION ENZYME, CONVENTIONAL, DRAI, 50U/UL,cuts TTT¬AAA at 37°C (suppliedwith 2mL of 10X

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 10243110

Quantity Price
1 £ 130.0 / 7500 units
EU Stock 3
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Description and Specification

Specification

Product Name Dral
Concentration 10U/µL
Incubator Temperature 37°C
Quantity 7,500U

5'...T T T▵A A A...3'

3'...A A A▵T T T...5'

Conditions for 100% Activity:

  • 1X Buffer Tango™:
  • 33mM Tris-acetate (pH7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • DraI is supplied in:10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with DraI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps — no effect
  • Dcm: never overlaps — no effect
  • CpG: never overlaps — no effect
  • EcoKI: may overlap — blocked
  • EcoBI: never overlaps — no effect

Digestion of Agarose-embedded DNA:

  • Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours