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Thermo Scientific™ CpoI (RsrII)

The CpoI (RsrII) restriction enzyme recognizes CG^GWCCG sites and cuts best at 37°C in Tango buffer (Isoschizomers: CspI, Rsr2I, RsrII).

Brand:  Thermo Scientific™ ER0742

UNSPSC : 12352204

Code : 88

Additional Details : Weight : 0.00900kg

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Product Code. 10528160

  • £120.00 / 1000 units


Estimated Shipment
08-09-2017
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Description

Description

5'...C G▵G W C C G...3'

3'...G C C W G▵G C...5'

Conditions for 100% Activity:

  • 1X Buffer Tango:33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • CpoI is supplied in:10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with CpoI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps-no effect
  • Dcm: never overlaps-no effect
  • CpG: completely overlaps-blocked
  • EcoKI: never overlaps-no effect
  • EcoBI: never overlaps-no effect

Compatible Ends:

  • Cfr13I, Eco47I, Psp5II, SanDI
Specifications

Specifications

CpoI (RsrII)
10U/µL
37°C
1000U