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Thermo Scientific™ BstXI

Cut at CCANNNNN^NTGG sites with BstXI restriction enzyme, which performs best at 55°C in O buffer.

Brand:  Thermo Scientific™ ER1021

UNSPSC : 12352204

Code : 88

Additional Details : Weight : 1.51950kg

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Product Code. 10171270

  • £19.48 / 500 units


EU Stock 8
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Specifications

Description

5'...C C A N N N N N▵N T G G...3'

3'...G G T N▵N N N N N A C C...5'

Conditions for 100% Activity:

  • b1X Buffer O: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 100mM NaCl and 0.1mg/mL BSA
  • Incubate at 55°C

Storage Buffer:

  • BstXI is supplied in: 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with BstXI, approximately 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps — no effect
  • Dcm: may overlap — cleavage impaired
  • CpG: never overlaps — no effect
  • EcoKI: never overlaps — no effect
  • EcoBI: never overlaps — no effect

Digestion of Agarose-embedded DNA:

  • Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours

     

Note:

Incubation at 37°C results in 50% activity. Greater than 15-fold overdigestion with BstXI may result in star activity. BstXI cleavage is impaired by overlappingdcm methylation. To avoiddcm methylation, use adam-, dcm strain such as GM2163 (#M0099).

Description & Specifications

Specifications

10U/µL
55°C
BstXI
500U