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Thermo Scientific™ Bsp1407I (BsrGI)

Cut at T^GTACA sites with Bsp1407I (BsrGI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BsrGI, BstAUI).

Brand:  Thermo Scientific™ ER0932

Code : 88

Additional Details : Weight : 0.00900kg

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Product Code. 10252920

  • £68.00 / 1500 units


EU Stock 10
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Description

Description

5'...T▵G T A C A...3'

3'...A C A T G▵T...5'

Conditions for 100% Activity:

  • 1X Buffer Tango™: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • Bsp1407I is supplied in:10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with Bsp1407I more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps — no effect
  • Dcm: never overlaps — no effect
  • CpG: never overlaps — no effect
  • EcoKI: never overlaps — no effect
  • EcoBI: never overlaps — no effect

Digestion of Agarose-embedded DNA:

  • Minimum 10units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours
  • Compatible Ends: Acc65I, BshNI, Pfl23II, TatI
Specifications

Specifications

Bsp1407I
10U/μL
37°C
300U
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