Thermo Scientific™ BseNI (BsrI)

Cut at ACTGG(1/-1)^ sites with BseNI (BsrI) restriction enzyme, which performs best at 65°C in B buffer (Isoschizomers: Bse1I, BsrI, BsrSI).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER0881

BSENI (BSRI) 10U/UL 1000U

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 10161270

Quantity Price
1 £ 51.75 / 1000 units
EU Stock 6
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Description and Specification

Specification

Product Name BseNI (BsrI)
Concentration 10U/µL
Incubator Temperature 65°C
Quantity 1000U

5'...A C T G G N▵...3'

3'...T G A C▵C N ...5'

Conditions for 100% Activity:

  • 1X Buffer B:10mM Tris-HCl (pH7.5 at 37°C), 10mM MgCl2 and 0.1mg/mL BSA
  • Incubate at 65°C
  • To ensure higher efficiency of digestion, perform the cleavage reaction under paraffin oil

Storage Buffer:

  • BseNI is supplied in:10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with BseNI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps — no effect
  • Dcm: never overlaps — no effect
  • CpG: never overlaps — no effect
  • EcoKI: may overlap — effect not determined
  • EcoBI: may overlap — effect not determined

Note:

Incubation at 37°C results in less than 10% activity.