Thermo Scientific™ BseGI (BtsCI)

Cut at GGATG(2/0)^ sites with BseGI (BtsCI) restriction enzyme, which performs best at 55°C in Tango buffer (Isoschizomers: BstF5I, BtsCI, FokI).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER0871

BSEGI (BTSCI) 10U/UL 500U

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 1.51950kg



Product Code. 10354340

Quantity Price
1 £ 14.530000000000001 / 500 units
EU Stock 19
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Description and Specification

Specification

Product Name BseGI (BtsCI)
Concentration 10U/µL
Incubator Temperature 55°C
Quantity 500U

5'...G G A T G N N▵...3'

3'...C C T A C▵N N ...5'

Conditions for 100% Activity:

  • 1X Buffer Tango™: 33mM Tris-acetate (pH7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 55°C

Storage Buffer:

  • BseGI is supplied in:10mM Tris-HCl (pH7.4 at 25°C), 100mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with BseGI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps — no effect
  • Dcm: may overlap — no effect
  • CpG: may overlap — no effect
  • EcoKI: never overlaps — no effect
  • EcoBI: may overlap — effect not determined

Note:

Incubation at 37°C results in 25% activity.