Thermo Scientific™ BseDI (BsaJI) (10 U/μL)

Cut at C^CNNGG sites with BseDI (BsaJI) restriction enzyme, which performs best at 55°C in Tango buffer (Isoschizomers: BsaJI, BssECI).

Brand:  Thermo Scientific™ ER1081

Product Code. 10689330

Description

Conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5'...C▵C N N G G...3'

3'...G G N N C▵C...5'

Conditions for 100% Activity:

• 1X Buffer Tango™: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
• Incubate at 55°C

Storage Buffer:

• BseDI is supplied in:10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

• After 50-fold overdigestion with BseDI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

• Dam: never overlap — no effect
• Dcm: may overlap — no effect
• CpG: may overlap — no effect
• EcoKI: never overlaps — no effect
• EcoBI: never overlaps — no effect

Note:

Incubation at 37°C results in 10% activity.

Specifications

• Concentration: 10 U/μL
• Enzyme: BseDI (BsaJI)
• Sensitive to Heat Inactivation: No
• Type IIS RE: No
• Product Type: Restriction Enzyme

• Methylation Sensitivity: Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
• Compatible Buffer: 10X Buffer Tango
• Optimal Reaction Temperature: 55°C
• Quantity: 300 U
• Research Category: Traditional Cloning

For Research Use Only. Not for use in diagnostic procedures.