BseDI (BsaJI)

Cut at C^CNNGG sites with BseDI (BsaJI) restriction enzyme, which performs best at 55°C in Tango buffer (Isoschizomers: BsaJI, BssECI).

Overview
Brand: Thermo Scientific

Manufacturer Part Number: ER1082

1500 UNIT RESTRICTION ENZYME, CONVENTIONAL, BSEDI(BsaJI), 10u/ul, cuts C¬CNNGG at 55°C (supplied

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.00900kg



Product Code. 10374630

Quantity Price
1 £ 42.46 / 1500 units
EU Stock 6
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Description and Specification

Specification

Product Name BseDI (BsaJI)
Concentration 10U/µL
Incubator Temperature 55°C
Quantity 1,500U

5'...C▵C N N G G...3'

3'...G G N N C▵C...5'

Conditions for 100% Activity:

  • 1X Buffer Tango™: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 55°C

Storage Buffer:

  • BseDI is supplied in:10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with BseDI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlap — no effect
  • Dcm: may overlap — no effect
  • CpG: may overlap — no effect
  • EcoKI: never overlaps — no effect
  • EcoBI: never overlaps — no effect

Note:

Incubation at 37°C results in 10% activity.