Thermo Scientific™ Bpu1102I (BlpI)

Cut at GC^TNAGC sites with Bpu1102I (BlpI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BlpI, Bsp1720I, CelII).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: ER0091

BPU1102I (BLPI) 10U/UL 200U

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 1.51950kg



Product Code. 10354450

Quantity Price
1 £ 26.43 / 200 units
EU Stock 9
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Description and Specification

Specification

Product Name Bpu1102l
Concentration 10U/µL
Incubator Temperature 37°C
Quantity 200U

5'...G C▵T N A G C...3'

3'...C G A N T▵C G...5'

Conditions for 100% Activity:

  • 1X Buffer Tango™: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • Bpu1102I is supplied in:10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol
  •  

Ligation and Recleavage:

  • After 50-fold overdigestion with Bpu1102I, more than 80% of the DNA fragments can be ligated in a reaction mixture containing 20-40 u of T4 DNA Ligase/1μg of fragments and 10% PEG. More than 95% of these can be recut

Methylation Effects:

  • Dam: never overlaps — no effect
  • Dcm: never overlaps — no effect
  • CpG: may overlap — no effect.
  • EcoKI: never overlaps — no effect.
  • EcoBI: may overlap — effect not determined

Digestion of Agarose-embedded DNA:

  • Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours
  • Compatible Ends: Bpu10I, Eco81I, HpyF3I