Learn More
Description
Thermo Scientific Biotin-11-dUTP (biotin-epsilon-aminocaproyl-[5-{3-aminoallyl}-2'-deoxyuridine-5'-triphosphate]) is supplied as 1 mM aqueous solution titrated to pH 7.0 with NaOH and is designed for enzymatic non-radioactive labelling of DNA.
Applications
• Enzymatic non-radioactive labeling of DNA by PCR
• Nick-translation
• cDNA synthesis
• Random primed labeling
• Primer extension
Biotin-11-dUTP can be enzymatically incorporated into DNA with:
• Reverse transcriptases
• Taq DNA Polymerase (Cat. No. EP0072)
• phi29 DNA Polymerase (EP0091)
• Klenow Fragment, exo (EP0421)
• Klenow Fragment (EP0051)
• DNA Polymerase I (EP0041)
Specifications
Specifications
| Concentration | 1 mM |
| Content And Storage | Biotin-11-dUTP Solution, 1 mM (50 μL) Store at –20°C. |
| Form | Liquid |
| Polymerase | DNA Polymerase |
| Labeling Method | Direct Labeling |
| Label or Dye | Biotin |
| Quantity | 50 μL |
| Shipping Condition | Dry Ice |
| For Use With (Application) | cDNA Synthesis, Non-Radioactive Labeling |
For Research Use Only. Not for use in diagnostic procedures.
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.