Thermo Scientific™ AarI

Cut at CACCTGC(4/8)^ sites with AarI restriction enzyme, which performs best at 37°C in its own unique (+oligo) buffer.

Brand: Thermo Scientific™

Manufacturer Part Number: ER1582


UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.01000kg

Product Code. 10282880

Quantity Price
1 £259.00 / 125 units
EU Stock 149
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Description and Specification


Product Name Aarl
Concentration 2U/µL
Incubator Temperature 37°C
Quantity 125U

5'...C A C C T G C (N)4▵...3'

3'...G T G G A C G (N)8▵...5'

Conditions for 100% Activity:

  • [1X Buffer AarI] + oligonucleotide:
  • [10mM Bis-Tris Propane-HCl (pH 6.5 at 37°C), 10mM MgCl2, 100mM KCl, 0.1mg/mL BSA] + 0.5μM of oligonucleotide (see Note)
  • Incubate at 37°C

Storage Buffer:

  • AarI is supplied in:
  • 10mM potassium phosphate (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 10-fold overdigestion with AarI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps — no effect
  • Dcm: never overlaps — no effect
  • CpG: may overlap — cleavage impaired
  • EcoKI: never overlaps — no effect
  • EcoBI: may overlap — effect not determined

Digestion of Agarose-embedded DNA:

  • Minimum 5 units of the enzyme are required for digestion of 1μg of agarose-embedded lambda DNA in 16 hours


For cleavage with AarI at least two copies of its recognition sequence are required. Inclusion of 0.5μM oligonucleotide with the AarI recognition sequence in the reaction mixture significantly improves cleavage of DNAs, especially of those with a single AarI site. Still, a complete cleavage of some substrates with AarI is difficult to achieve. Greater than 10-fold overdigestion with AarI may result in star activity. AarI may remain associated with the cleaved DNA. This may cause DNA band shifting during electrophoresis. To avoid atypical DNA band patterns, use the 6X DNA Loading Dye & SDS Solution for sample preparation or heat the digested DNA in the presence of SDS prior to electrophoresis.