FceR1 alpha Monoclonal Antibody (MAR-1), Functional Grade, eBioscience™, Invitrogen™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-5898-025

Product Code. 17121352

Description

The MAR-1 MAb reacts with the Fc epsilon Receptor I alpha subunit, an IgE-binding subunit lacking signal-transducing ability. Fc epsilon RI alpha; is expressed on mast and basophil cells and is up-regulated by the presence of IgE. Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits. The beta and gamma subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The Fc epsilon RI complex plays an important role in triggering IgE-mediated allergic reactions. Applications Reported: The MAR-1 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking of IgE to Fc epsilon Receptor and degranulation of mast cells. Applications Tested: The MAR-1 antibody has been tested by flow cytometric analysis of the MC/9 cell line (a mouse mast cell line). This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/ μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

FceR1 alpha Monoclonal antibody specifically detects FceR1 alpha in Mouse samples. It is validated for Flow Cytometry, Functional Assay, Neutralization

Specifications

• Antigen: FceR1 alpha
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: P20489
• Host Species: Armenian Hamster
• Quantity: 25 mg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG

• Applications: Flow Cytometry, Functional Assay, Neutralization
• Clone: MAR-1
• Conjugate: Functional Grade
• Gene: FCER1A
• Gene Alias: fc epsilon r1; Fc epsilon receptor Ia; Fc epsilon RI alpha-chain; Fc fragment of IgE receptor Ia; Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide; Fc IgE receptor, alpha polypeptide; Fc receptor, IgE, high affinity I, alpha polypeptide; Fce1a; Fc-epsilon RI-alpha; fcepsilonri; FCER1A; FcERI; Fcr-5; high affinity immunoglobulin epsilon receptor alpha-subunit; high affinity immunoglobulin epsilon receptor subunit alpha; IgE Fc receptor subunit alpha; immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide; RP11-550P17.3
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 14125
• Content And Storage: 4° C
• Form: Liquid