FceR1 alpha, PerCP-eFluor™ 710, clone: MAR-1, eBioscience™
Armenian Hamster Monoclonal Antibody
Brand: Affymetrix eBioscience 46-5898-82
Code : NEW
Additional Details : Weight : 0.09500kg
DescriptionDescription: The MAR-1 monoclonal antibody reacts with the Fc epsilon Receptor I alpha subunit, an IgE-binding subunit lacking signal-transducing ability. Fc epsilon RI alpha is expressed on mast and basophil cells and is up-regulated by the presence of IgE. Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits. The beta and gamma subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The Fc epsilon RI complex plays an important role in triggering IgE-mediated allergic reactions.Applications Reported: This MAR-1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This MAR-1 antibody has been tested by flow cytometric analysis of MC/9 cell line (a mouse mast cell line). This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
|Fc epsilon Receptor 1 alpha (FceRI)|
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|RP11-550P17.3, FCE1A, FcERI|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.