Thermo Scientific™ FastDigest™ SmaI

The FastDigest SmaI restriction enzyme recognizes CCC^GGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: TspMI, XmaCI; neoschizomer: XmaI)

Brand: Thermo Scientific™

Manufacturer Part Number: FD0663

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.01000kg

Product Code. 10110890

Quantity Price
1 £ 24.53 / 100 reactions
EU Stock 23
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Description and Specification


For Use With (Application) Fast clone analysis; Fast preparation of DNA for cloning; Digestion of PCR products; Fast RFLP genotyping; Digestion of difficult-to-cleave DNA
Quantity 100µL
Components 10X FastDigest Buffer and 10X FastDigest Green Buffer
Product Name FastDigest Smal


  • 100% activity of all FastDigest enzymes in the universal buffer
  • 100% buffer compatibility with downstream applications
  • Complete digestion in 5-15 minutes
  • Direct loading on gels
  • No star activity
  • 176 FastDigest enzymes available


  • Molecular cloning
  • Restriction site mapping
  • Genotyping
  • Southern Blot
  • Restriction fragment length polymorphism (RFLP)
  • SNP analysis


The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.

For methylation sensitivity refer to product specifications.