Thermo Scientific™ FastDigest™ SacI

The FastDigest SacI restriction enzyme recognizes GAGCT^C sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Eco53kI, EcoICRI, Psp124BI, SstI)

Brand: Thermo Scientific™

Manufacturer Part Number: FD1134

200 UL RESTRICTION ENZYME, FASTDIGEST, SACI, CUTSGAGCT¬C, 1µL cleaves 1µg DNA in 5 minutes at 37°C

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.01000kg

Product Code. 10889180

Quantity Price
1 £45.77 / 200 reactions
Estimated Shipment date
from Supplier 01-11-2016
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Description and Specification


Product Name FastDigest SacI
Quantity 200µL (200 reactions)


  • 100% activity of all FastDigest enzymes in the universal buffer
  • 100% buffer compatibility with downstream applications
  • Complete digestion in 5-15 minutes
  • Direct loading on gels
  • No star activity
  • 176 FastDigest enzymes available


  • Molecular cloning
  • Restriction site mapping
  • Genotyping
  • Southern Blot
  • Restriction fragment length polymorphism (RFLP)
  • SNP analysis


The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.

For methylation sensitivity refer to product specifications.