Invitrogen™ FAM21A Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA561604

Product Code. 15789411

Description

Immunogen sequence: VRVYDEEVEE PVLKAEAEKT EQEKTREQKE VDLIPKVQEA VNYGLQVLDS AFEQLDIKAG NSDSEEDDAN GRVELILEPK DLYIDRPLPY LIGSKLFME Highest antigen sequence identity to the following orthologs: Mouse - 94%, Rat - 91%.

Acts at least in part as component of the WASH core complex whose assembly at the surface of endosomes inhibits WASH nucleation-promoting factor (NPF) activity in recruiting and activating the Arp2/3 complex to induce actin polymerization and is involved in the fission of tubules that serve as transport intermediates during endosome sorting. Mediates the recruitment of the WASH core complex to endosome membranes via binding to phospholipids and VPS35 of the retromer CSC. Mediates the recruitment of the F-actin-capping protein dimer to the WASH core complex probably promoting localized F-actin polymerization needed for vesicle scission. Via its C-terminus binds various phospholipids, most strongly phosphatidylinositol 4-phosphate (PtdIns-(4)P), phosphatidylinositol 5-phosphate (PtdIns-(5)P) and phosphatidylinositol 3,5-bisphosphate (PtdIns-(3,5)P2). Involved in the endosome-to-plasma membrane trafficking and recycling of SNX27-retromer-dependent cargo proteins, such as GLUT1. Required for the association of DNAJC13, ENTR1, ANKRD50 with retromer CSC subunit VPS35. Required for the endosomal recruitment of CCC complex subunits COMMD1 and CCDC93 as well as the retriever complex subunit VPS35L. [UniProt].

Specifications

• Antigen: FAM21A
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: WASHC2A
• Gene Alias: alternative protein FAM21B; bA56A21.1; bA98I6.1; FAM21A; FAM21B; family with sequence similarity 21 member A; family with sequence similarity 21, member A; family with sequence similarity 21, member B; WASH complex subunit 2A; WASH complex subunit FAM21A; WASH complex subunit FAM21B; WASHC2A
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 387680
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Immunocytochemistry
• Concentration: 0.2 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q641Q2
• Gene Symbols: WASHC2A
• Immunogen: Recombinant protein corresponding to Human FAM21A. Recombinant protein control fragment (Product #RP-104434).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG