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Gibco™ ExpiSf™ CD Medium
Description
- Delivers high reproducibility and lot-to-lot reliability
- Facilitates high-level expression of baculovirus-infected cultures, achieving protein yields three times higher than traditional insect expression platforms
- Supports growth of suspension Sf9 cultures to densities of approximately 2 x 107 cells/mL, with >90% cell viability
- Supports high-density infection of suspension Sf9 cells with recombinant baculovirus
- Supports growth, transfection, and baculovirus infection of Sf9 cells in culture formats from 24-deep well plates to >3L in shaker flasks
- Is a ready-to-use formulation, with no supplementation required
- manufactured using an ISO 13485-certified process according to 21 CFR 820 medical device current good manufacturing practices (cGMP)
ExpiSf™ CD Medium is a core component of the ExpiSf Expression System. For highest level protein expression, the medium is used in combination with a matched protein expression enhancer, ExpiSf Enhancer, which is supplied as part of the ExpiSf Protein Production Kit (Cat. No. A3767806). Sf21 and High Five™ cells adapted to other commercially-available serum-free media may be adapted for growth in ExpiSf CD Medium; although, sequential adaptation is required and high-density growth isn't guaranteed. ExpiSf™ CD Medium is not recommended for adherent cell cultures. Cells are typically grown in Erlenmeyer-style flasks on a shaker platform in a non-humidified, non-CO2 cell culture incubator.
Specifications
Specifications
| Quantity | 500 mL |
| Content And Storage | 1 x 500 mL bottle, store in the dark at 2–8°C Shelf Life: 12 months from date of manufacture |
| Classification | Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free |
| Compatible Cells | Insect Cells |
| Format | Liquid |
| Shipping Condition | Room Temperature |
| Product Line | ExpiSf |
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