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Description
- ExoSAP-IT™ PCR Product Cleanup Reagent is used for enzymatic cleanup of amplified PCR product by hydrolyzing excess primers and nucleotides in a single step
- ExoSAP-IT-purified samples are ready for use in downstream applications such as DNA sequencing or single nucleotide polymorphism (SNP) analysis
- The reaction setup is complete with one pipetting step, which is followed by two incubations
- The first incubation digests excess primer and dephosphorylates nucleotides
- The second, high-temperature incubation inactivates the enzymes
- Adding ExoSAP-IT reagent directly to the PCR product eliminates transfer steps to tubes, wells, or columns and conserves PCR amplicons, helping reduce the chance of cross contamination
- After enzymatic cleanup with ExoSAP-IT reagent, primers and dNTPs will no longer interfere with DNA sequencing or SNP analysis.
Specifications
Specifications
| Content And Storage | Store at -5°C to -30°C. |
| Format | Kit |
| Sample Type | PCR Products |
| For Use With (Application) | PCR Purification |
| No. of Reactions | 100 Preps |
| Protein Form | Recombinant |
| Quantity | 200 μL, 100 Preps |
| Shipping Condition | Dry Ice |
Product Title
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