Invitrogen™ Estrone-3-Glucuronide (E1G) Competitive ELISA Kit

Product Code. 15805853

Brand: Invitrogen™ EIA17E3

Description

ELISA

The Estrone-3 Glucuronide (E1G) ELISA quantitates estrone-3 glucuronide in extracted serum,plasma,urine,dried fecal extracts or cell culture medium.

Principle of the method

The Estrone-3-Sulfate (E1S) Competitive ELISA research-use-only kit uses a specifically generated antibody to measure Estrone-3-Sulfate (E1S) in a variety of matrices,including serum,plasma,urine,and fecal samples. The kit will quantitatively measure E1S present in diluted buffer samples and tissue culture media samples. An E1S standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An E1S-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to E1S to each well. After a 2-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound E1S-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.

Estrone-3-glucuronide (E1G) is the principle secreted form of circulating estradiol in mammals. Estrone-3-glucuronide (E1G) is identical across all species.

Specifications

• Assay Range: 15.6 to 1000 pg/mL
• Assay Sensitivity: 7.38 pg/mL
• Description: Estrone-3-Glucuronide (E1G) Competitive ELISA Kit
• Assay: Multiplex assay on the Luminex Platform
• Conjugate: HRP
• Detection Method: Fluorescence
• Product Type: ELISA
• Protein Family: Steroid hormone
• Sample Type: Fecal Extract, Serum, Supernatant, Urine
• Sufficient For: 96 assays
• Target Species: Human
• For Use With (Application): ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
• For Use With (Equipment): Colorimetric Microplate Reader
• Interassay CV: 5.6%
• Intraassay CV: 3.8
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, HRP-Conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Research Discipline: Reproductive Biology
• Sample Volume: Fecal Extract 50 μL, Serum 50 μL, Supernatant 50 μL, Urine 6.25 μL
• Storage Requirements: -20°C
• Target: Estrone-3-Glucuronide
• Test Time: 1 hrs 20 mins
• Total Assay Time: 2 hrs 30 mins