Estrogen Receptor alpha Monoclonal Antibody (33), Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen MA1310

Product Code. 11569602

Description

MA1-310 detects both the steroid occupied and unoccupied forms of the estrogen receptor (ER) alpha from human samples. MA1-310 detects ER alpha and does not cross react with ER beta. MA1-310 has been successfully used in Western blot, immunohistochemistry, immunoprecipitation, gel shift, flow cytometry, and immunocytochemical experiments. By Western blot, this antibody detects an ∽66 kDa protein representing ER alpha from MCF-7 cell extract. MA1-310 can be used with blocking peptide PEP-013.

Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.

Specifications

• Antigen: Estrogen Receptor alpha
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: Dulbecco's PBS with 1mg/mL BSA, 30% glycerol and 0.05% sodium azide
• Gene Accession No.: P03372, P06211, P19785, P49884
• Gene Symbols: ESR1
• Immunogen: Synthetic peptide corresponding to the residues E(247) V G M M K G G I R K D R R G(261) of the ER alpha DNA binding domain.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Bovine, Human, Mouse, Non-human Primate, Rat
• Product Type: Antibody
• Isotype: IgG1

• Applications: Immunocytochemistry, Immunohistochemistry, Western Blot
• Clone: 33
• Conjugate: Unconjugated
• Gene: ESR1
• Gene Alias: DKFZp686N23123; ER; Er alpha; ER36; ERa; ERalpha; ER-alpha; ERalpha protein; ESR; esr 1; Esr1; esr-1; ESRA; Estr; Estra; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor 1; estrogen receptor 1 (alpha); estrogen receptor alpha; estrogen receptor alpha c-terminus splice variant 1-2; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha splice variant, CTERP-1; estrogen receptor alpha splice variant, ERalphaDup5; estrogen receptor alpha splice variant, ERalphai45a; estrogen receptor alpha splice variant, ERalphai45bL; estrogen receptor alpha splice variant, ERalphai45bS; estrogen receptor alpha splice variant, ERalphai45c; estrogen receptor alpha splice variant, ERalphai56; estrogen receptor alpha splice variant, ERalphai67; estrogen receptor alpha variant delta 4; estrogen receptor protein; estrogen receptor, alpha; ESTRR; hER-alpha36; I79_001166; Nr3a1; nuclear receptor; nuclear receptor subfamily 3 grou
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 13982, 2099, 24890, 407238
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Liquid