Invitrogen™ Human Estradiol E2 ELISA Kit

Product Code. 18466798

Brand: Invitrogen™ KAQ0622

Description

ELISA

The Human Estradiol E2 ELISA Kit is a solid-phase sandwich Enzyme-Linked Immunosorbent Assay (ELISA). This assay is designed to detect and quantify the level of 17-beta-estradiol (E2) in serum and plasma. The assay will recognize both natural and recombinant human estradiol E2. Principle of the method The Human Estradiol solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Estradiol (17 beta-estradiol) is a sex hormone. Estradiol is the predominant sex hormone present in females. It is also present in males, being produced as an active metabolic product of testosterone. It represents the major estrogen in humans. Estradiol has not only a critical impact on reproductive and sexual functioning, but also affects other organs including the bones.

Specifications

• Assay Range: 25-2000 pg/mL
• Assay Sensitivity: 10.6 pg/mL
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Hormones & Receptors
• Sample Type: Plasma, Serum
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 17-Beta-Estradiol; 17-beta-HSD17-Beta-Estradiol; 17-beta-HSD;
• Interassay CV: 8.9%
• Intraassay CV: 11%
• Kit Contents: Pre-coated 96 well strip-well plate, Standard, Controls, SAV-HRP, Wash Buffer, Chromogen, Stop Solution
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Shipping Condition: Wet Ice
• Storage Requirements: 2-8°C
• Target: Estradiol
• Test Time: 1 hr 20 min
• Total Assay Time: 2 hr