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ERK1/2, eFluor 570, clone: 5AD13MA, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 41-9108-82

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15508956

  • £167.61 / 100µg

Estimated Shipment date
from Supplier 25-04-2018
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Description: The 5AD13MA monoclonal antibody recognizes human, mouse, and rat extracellular signal-regulated kinases 1 and 2 (also known as ERK1/2, p44/42, or MAPK3/1). ERK1/2 belong to a family of conserved serine/threonine protein kinases known as mitogen-activated protein kinases (MAPKs) that are involved in many cellular programs such as proliferation, differentiation, motility, and survival. The ERK1/2 signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines. The primary activators of ERK1/2 are MEK1 and MEK2 which activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187 in ERK1 and ERK2, respectively. Several downstream targets of ERK1/2 have been identified, including p90RSK and the transcription factor Elk-1. ERK1/2 are negatively regulated by MAPK phosphatases as well as by chemical inhibitors of MEK including U0126 and PD98059. The ERK pathway is disrupted in many types of cancer and thus is an important target for diagnosis and treatment.Applications Reported: This 5AD13MA antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.Applications Tested: This 5AD13MA antibody has been tested by immunocytochemistry of methanol-fixed cells and can be used at less than or equal to 5μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.Excitation: 555 nm; Emission: 570 nm


ERK 1/2
eFluor 570
Store at 2-8°C. Do not freeze. Light-sensitive material.
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
MAPK, ERK, p42/p44
IgG1, kappa

For Research Use Only.