PeproTech Eotaxin 3 Polyclonal Antibody, PeproTech®, Invitrogen™

Goat Polyclonal Antibody

Brand:  PeproTech 500-P156G-50UG

Product Code. 17863843

Description

AA Sequence of recombinant protein: TRGSDISKTC CFQYSHKPLP WTWVRSYEFT SNSCSQRAVI FTTKRGKKVC THPRKKWVQK YISLLKTPKQ L. Preparation: Produced from sera of goats immunized with highly pure Recombinant Human Eotaxin-3 (CCL26). Anti-Human Eotaxin-3 (CCL26)-specific antibody was purified by affinity chromatography employing an immobilized Human Eotaxin-3 (CCL26) matrix. Sandwich ELISA: To detect Human Eotaxin-3 (CCL26) by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Eotaxin-3 (CCL26) (500-P156GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Eotaxin-3 (CCL26). Western Blot: To detect Human Eotaxin-3 (CCL26) by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Eotaxin-3 (CCL26) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Eotaxin-3 is a small cytokine belonging to the CC chemokine family that is also called CCL26, Chemokine (C-C motif) ligand 26. Chemokines play pivotal roles in health and disease by controlling migration and tissue localization of specific types of cells expressing their cognate receptors. Eotaxin-3 is chemotactic for eosinophils and basophils and elicits its effects by binding to the cell surface chemokine receptor CCR3. It is expressed by several tissues including heart, lung and ovary, and in endothelial cells that have been stimulated with the cytokine interleukin 4. This gene for chemokine is located on human chromosome 7. Eotaxin-3 induced migration of eosinophils and basophils only at a 10-fold higher concentration than eotaxin.

Specifications

• Antigen: Eotaxin 3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CCL26
• Gene Alias: CC chemokine IMAC; C-C motif chemokine 26; C-C motif chemokine ligand 26; Ccl26; Ccl26l; chemokine (C-C motif) ligand 26; chemokine N1; Eotaxin3; eotaxin-3; IMAC; Macrophage inflammatory protein 4-alpha; MGC126714; MIP-4a; MIP-4alpha; MIP-4-alpha; PRO242; SCYA26; SCYA26MIP-4-alpha; small inducible cytokine A26; small inducible cytokine subfamily A (Cys-Cys), member 26; small-inducible cytokine A26; Thymic stroma chemokine-1; TSC-1; UNQ216; UNQ216/PRO242
• Host Species: Goat
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 10344
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: Q9Y258
• Gene Symbols: CCL26
• Immunogen: E.coli-derived Recombinant Human Eotaxin-3 (CCL26).
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody