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eNOS Polyclonal Antibody, Invitrogen™
Rabbit Polyclonal Antibody
Brand: Invitrogen PA137625
Code : LD
Additional Details : Weight : 0.01000kg
Description
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is placenta, lung or ovarian carcinoma.
Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.Specifications
eNOS | |
Polyclonal | |
PBS with 1% BSA and 0.1% sodium azide; pH 7.6 | |
P29474 | |
NOS3 | |
Synthetic peptide from C-terminus of human e-NOS | |
1 mL | |
Primary | |
Human | |
Antibody | |
IgG |
Immunohistochemistry (Paraffin) | |
Unconjugated | |
NOS3 | |
2310065A03Rik; cNOS; Constitutive NOS; Ecnos; EC-NOS; endothelial nitric oxide synthase; endothelial nitric oxide synthase 3; endothelial nitric oxide synthase NOS3; endothelial NOS; eNos; e-NOS; nitric oxide synthase; nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell); nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 transcript variant eNOS-delta21; nitric oxide synthase 3, endothelial cell; nitric oxide synthase, endothelial; NOS; NOS type III; Nos3; Nos-3; NOSIII; OTTHUMP00000213183 | |
Rabbit | |
Antigen affinity chromatography | |
RUO | |
4846 | |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. | |
Liquid |