EMI1 Antibody (EMI1/1176), Alexa Fluor™ 532, Novus Biologicals™

Mouse Monoclonal Antibody

Brand:  Novus Biologicals NBP2-47786AF532

Product Code. 18131269

Description

EMI1 Monoclonal specifically detects EMI1 in Human samples. It is validated for Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: EMI1
• Classification: Monoclonal
• Conjugate: Alexa Fluor 532
• Formulation: 50mM Sodium Borate with 0.05% Sodium Azide
• Gene Symbols: FBXO5
• Immunogen: Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein (Uniprot: Q9UKT4)
• Quantity: 0.1 mL
• Primary or Secondary: Primary
• Target Species: Human
• Isotype: IgG2a κ

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunofluorescence
• Clone: EMI1/1176
• Dilution: Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunofluorescence
• Gene Alias: EMI1Early mitotic inhibitor 1, F-box only protein 5, F-box protein 5, FBX5F-box protein Fbx5, Fbxo31
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Test Specificity: It recognizes a 56kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.
• Content And Storage: Store at 4°C in the dark.