Western blotting is a commonly used technique for studying protein function and localization. Typically, protein samples are electrophoresed on SDS-PAGE and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid-based technologies, which allow reuse of Southern and Northern blots, it has been difficult to reuse Western blots.
No pungent-smelling β-mercaptoethanol is contained in the Antibody Stripping Solution
Antibody stripping is done at room temperature—no heating of blots is required