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Merck ReBlot Plus Kit and Reagents
Description
Includes
Mild Antibody Stripping Solution (10X), 50mL; Strong Antibody Stripping Solution (10X), 50mL
Western blotting is a commonly used technique for studying protein function and localization. Typically, protein samples are electrophoresed on SDS-PAGE and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid-based technologies, which allow reuse of Southern and Northern blots, it has been difficult to reuse Western blots.
- No pungent-smelling β-mercaptoethanol is contained in the Antibody Stripping Solution
- Antibody stripping is done at room temperature—no heating of blots is required
- Blots may be reused in 25 min.
Specifications
Specifications
| Product Type | Mild WB Stripping Buffer |
| For Use With (Application) | Western Blot |
| Quantity | 50 mL |
| Concentration | 2X |
| Kit Contents | 25mL Mild Antibody stripping solution 10X |
| Content And Storage | 2°C to 8°C |
Safety and Handling
- Re-Blot Plus Mild Solution (10X)
- Danger
- Acute toxicity Category 3
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 2
- Substances and mixtures corrosive to metals Category 1
- Skin corrosion/irritation Category 1 A
- H302-Harmful if swallowed.
- H311-Toxic in contact with skin.
- H314-Causes severe skin burns and eye damage.
- H332-Harmful if inhaled.
- H411-Toxic to aquatic life with long lasting effects.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P308-IF exposed or concerned:.
- MIXTURE LIST-Contain: sodium hydroxide, sodium azide
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