EMD Millipore Luminata™ Western HRP Chemiluminescence Substrates

Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns

Overview
Brand: EMD Millipore

Manufacturer Part Number: WBLUC0500

UNSPSC: 41105300

Code: 72

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 11770644

Quantity Price
1 £ 218.0 / 500mL
In Stock 7
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Description and Specification

Specification

Sensitivity ∽6pg
Storage Requirements 2° to 8°C
Applications Western blotting, dot/slot blotting
Product Type Luminata Classico Western HRP Substrate
Format HRP
Quantity 500mL

Horseradish peroxidase (HRP) catalyzes the oxidation of luminol in the presence of peroxide, releasing photons (chemiluminescence) that are captured on x-ray films or by CCD cameras. Most HRP substrates are plagued by quickly decaying signal and short shelf life, but Luminata substrates are formulated differently to include stabilizing reagents.

  • Consistent results with less pipetting error

The stabilizers added to Luminata substrates:

  • Prevent spontaneous oxidation of luminol by peroxide in the absence of HRP
  • Enhance the stability of both the luminol and peroxide, ensuring that Luminata substrates are stable for one year in their premixed format
  • Stabilize, strengthen, and prolong the chemiluminescence signal once HRP starts to catalyze oxidation

Three different sensitivities for a range of detection needs:

  • Luminata Classico premixed reagent for everyday detection needs
  • Luminata Crescendo premixed reagent for detection in the picogram range
  • Luminata Forte premixed reagent for detection in the femtogram range