Invitrogen™ Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit

These complete, ready-to-use kits include clear 96-well plate(s), other assay specific reagents, buffers, and a lot-specific technical data sheet.

Brand:  Invitrogen™ EEA022

Product Code. 17992141

Description

The Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit is a highly reliable and sensitive assay designed for the quantitative measurement of T-AOC in serum, plasma, whole blood, tissue, cells, and culture supernatant. T-AOC refers to the overall ability of a biological sample to counteract oxidative stress by neutralizing reactive oxygen species (ROS) and other harmful free radicals. It represents the cumulative effect of both enzymatic and non-enzymatic antioxidants present in the sample.

The assay quantifies the ability of the sample to reduce ferric ions (Fe³⁺) to ferrous ions (Fe²⁺) using a phenanthroline substance. The resulting complex exhibits a characteristic absorbance at 520 nm, which is directly proportional to the T-AOC of the sample.

Key features
• Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period
• Broad range of kits—across key analytes and targets within the most important metabolic pathways
• Consistent—strict quality control criteria to ensure reproducible and reliable results

Specifications

• Assay Range: 0.62–190.43 U/mL
• Content And Storage: Store at 2–8°C.
  
  
• Sample Type: Cell Culture Supernatant, Plasma, Serum, Tissue Homogenates, Whole Blood, Whole Cells
• Incubation Time: 40 min.
• Intraassay CV: 4.8
• Shipping Condition: Dry Ice, 2–8°C
• Total Assay Time: 50 min.

• Assay Sensitivity: 0.62 U/mL
• Detection Method: Colorimetric
• For Use With (Equipment): Microplate Reader
• Interassay CV: 5.6
• Sample Volume: 20 μL
• Target Kit Named: Total Antioxidant Capacity (T-AOC)