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Invitrogen™ Low-density Lipoprotein Cholesterol (LDL-C) Colorimetric Assay Kit

Product Code. 17972171
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Quantity:
96 tests
Unit Size:
Pack of 96
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Product Code. Quantity unitSize
17972171 96 tests Pack of 96
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Product Code. 17972171 Supplier Invitrogen™ Supplier No. EEA014

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Description

These complete, ready-to-use kits include clear 96-well plate(s), other assay specific reagents, buffers, and a lot-specific technical data sheet.

The Low-Density Lipoprotein Cholesterol (LDL-C) Colorimetric Assay Kit is a highly reliable and sensitive assay designed for the quantitative measurement of LDL-C in serum, plasma, tissue, cells, and cell culture supernatant samples. LDL-C is the main lipoprotein in fasting plasma, accounting for about two-thirds of plasma lipoproteins, and is the main vehicle for transporting cholesterol to extrahepatic tissues. The content of LDL-C is related to the incidence of cardiovascular disease and the degree of lesions and is considered to be the main pathogenic factor of atherosclerosis.

The assay is based on the reaction of LDL-C with enzyme reagents containing cholesterol esterase and cholesterol oxidase to produce hydrogen peroxide. Hydrogen peroxide is catalyzed by an oxidase in the presence of 4-aminoantipyrine (4-AA) and phenol (T-OOS) to form a red quinone compound with a maximum absorption peak at 546 nm. The LDL-C content in the sample can be calculated by measuring the OD value.

Key features
• Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period
• Broad range of kits—across key analytes and targets within the most important metabolic pathways
• Consistent—strict quality control criteria to ensure reproducible and reliable results

TRUSTED_SUSTAINABILITY

Specifications

Assay Range 0.2–12 mmol/L
Assay Sensitivity 0.2 mmol/L
Content And Storage Store at 2–8°C.
Detection Method Colorimetric
Sample Type Cell Culture Supernatant, Plasma, Serum, Tissue Homogenates, Whole Cells
For Use With (Equipment) Microplate Reader
Incubation Time 10 min.
Interassay CV 10
Intraassay CV 5.5
Quantity 96 tests
Sample Volume 2.5 μL
Shipping Condition Dry Ice, 2–8°C
Target Kit Named Low-density Lipoprotein Cholesterol (LDL-C)
Total Assay Time 20 min.
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